Journal: International Journal of Surgery (London, England)
Article Title: The microbial composition of pancreatic ductal adenocarcinoma: a systematic review of 16S rRNA gene sequencing
doi: 10.1097/JS9.0000000000001762
Figure Lengend Snippet: Summary of included studies.
Article Snippet: Comparisons of oral, intestinal, and pancreatic bacterial microbiomes in patients with pancreatic cancer and other gastrointestinal diseases. Chung M et al . 2021. USA , PDAC ( n =24) Ampullary adenocarcinoma ( n =8) Cholangiocarcinoma ( n =4) Benign controls ( n =16) 6 normal pancreatic tissues 33 pancreatic tumour samples 22 Duodenum tissue 316 oral samples (52 tongue swab, 46 buccal swab, 35 supragingival swab, 48 saliva samples) 34 jejunum swab, 19 bile duct swab samples, 21 pancreatic ducts, , 16S rRNA gene analysis performed V3-V4 hypervariable regions of the 16SrRNA gene. Sequence quality checking and denoising were performed using the DADA2 Illumina sequence denoising process. Taxonomic classification, alignment, and phylogenetic tree building were completed using QIIME2. , Fusobacterium nucleatum was among the top shared species based on the taxonomic annotations between oral and intestinal or pancreatic samples. ASV (Amplicon Sequence Variants) that were present in PDAC were: Fusobacterium Rothia Saccharibacteria Oribacterium Streptococcus , Not mentioned , Oral, intestinal, and pancreatic bacterial microbiomes overlap but exhibit distinct co-abundance patterns in patients with PDAC and other gastrointestinal diseases. , Yes, available. PRJNA558364..
Techniques: Sequencing, Positive Control, Clinical Proteomics, Bacteria, Control, Biomarker Discovery, Generated, Extraction, Transmission Assay, Software, Amplification, Formalin-fixed Paraffin-Embedded, Diagnostic Assay, Marker, Construct, Transplantation Assay